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KMID : 1145120160140040449
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Asian Journal of Beauty and Cosmetology
2016 Volume.14 No. 4 p.449 ~ p.461
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Availability of Opuntia ficus-indica var. saboten Stem Extracts as a Natural Preservative
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Lim Mi-Hye
Hong Se-Jeong
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Abstract
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Purpose: This study was carried out to develop natural cosmetic preservatives in replace of the chemical preservatives.
Methods: The extracts of Opuntia ficus-indica var. saboten stem (OFS), which is known for its excellent anti-oxidant properties, were obtained by reflux extraction with 80% ethanol. Its anti-microbial activity was tested using minimal inhibition concentration (MIC) test and its preservative activity was tested by challenge test in mud pack.
Results: As a result, the MIC of OFS for each strain represented 12.5% of Pseudomonas aeruginosa (P. aeruginosa), 12.5% of Staphylococcus aureus (S. aureus), 1.5% of Escherichia coli (E. coli), 20% of Candida albicans (C. albicans), and 20% of Aspergillus niger (A. niger). At this time, MIC of 1,2-hexanediol showed 1% of P. aeruginosa, 2.5% of S. aureus, 1.5% of E. coli, 1% of C. albicans, and 5% of A. niger. In the containing 20% of OFS mud pack, P. aeruginosa, S. aureus, and E. coli were decreased approximately 99.9999% within 2nd day and this trend was maintained up to 28th day. C. albicans was decreased approximately 99.99% within 14th day and this trend was also maintained up to 28th day. A. niger, on the other hand, was rapidly decreased within the 21st and the 28th day. The results demonstrated that containing 20% of OFS mud pack has a preservative activity in accordance to the standards of European Pharmacopoeia (EP), United States Pharmacopoeia (USP) and Cosmetic, Toiletry, and Fragrance Association (CTFA).
Conclusion: Based on the result, if OFSs were to be applied to cosmetics as natural preservatives in the future, it would provide a high commercial value.
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KEYWORD
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Opuntia ficus-indica var. saboten stem extracts, Cosmetics, Minimal inhibition concentration, Challenge test, Natural preservative
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